LOCUS BI916519 589 bp mRNA linear EST 16-OCT-2001 DEFINITION 603178420F1 NIH_MGC_121 Homo sapiens cDNA clone IMAGE:5242780 5', mRNA sequence. ACCESSION BI916519 VERSION BI916519.1 GI:16180488 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 589) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11612 row: g column: 05 High quality sequence stop: 587. FEATURES Location/Qualifiers source 1..589 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:5242780" /clone_lib="NIH_MGC_121" /lab_host="DH10B" /note="Organ: brain; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source anonymous pool of 3 fetal brains, female age 20 weeks, female age 24 weeks, and male age 26 weeks. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7 kb, insert size range 0.7-3.5 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 017. Note: this is a NIH_MGC Library." BASE COUNT 111 a 166 c 178 g 132 t 2 others ORIGIN 1 gcttcctcag gcagcgggag ggccagttca gcagcctgca gctggtggcc atgcagcggg 61 gaggtggctg ctgccatgca gtacctgtcc agctttgcct tcgtccatcg ctcgctgtct 121 gcccacagcg tgctggtgaa tagccacttg gtgtgcaagg tggcccgtct tggccacagt 181 cctcagggcc caagttgttt gcttcgctgg gcagccccag aggtcattgc acatggaaag 241 catacaacat ccagtgatgt ctggagcttt gggatactca tgtgggaagt gatgagttat 301 ggagaacggc cttactggga catgagtgag caggaggtac taaatgcaat agagcaggag 361 ttccggctgc ccccgcctcc aggctgtcct cctggattac atctacttat gttggacact 421 tggcagaagg accgtgcccg gcggnctcat tttgaccagc tggtggctgc atttgacaag 481 atgatccgca agccagatac cctgcaggct ggcggggacc cangggaaag gccttcccag 541 gcccttctga cccctgtggc cctggacttt ccttgtctgg actcaccca // LOCUS BI913359 885 bp mRNA linear EST 16-OCT-2001 DEFINITION 603178843F1 NIH_MGC_121 Homo sapiens cDNA clone IMAGE:5243122 5', mRNA sequence. ACCESSION BI913359 VERSION BI913359.1 GI:16177732 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 885) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11613 row: e column: 11 High quality sequence stop: 826. FEATURES Location/Qualifiers source 1..885 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:5243122" /clone_lib="NIH_MGC_121" /lab_host="DH10B" /note="Organ: brain; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source anonymous pool of 3 fetal brains, female age 20 weeks, female age 24 weeks, and male age 26 weeks. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7 kb, insert size range 0.7-3.5 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 017. Note: this is a NIH_MGC Library." BASE COUNT 151 a 276 c 281 g 177 t ORIGIN 1 cgggcccgga ctgctgccgg ccacggcccc tacggcgtgc aagtctattt ccagacactt 61 cctcacgggg agctgtcttc ccagcttccg gaaagactct ccttggtgat cggctccatc 121 ctgggggctt tggccttcct cctgctggca gccatcaccg tgctggcggt cgtcttccag 181 cggaagcggc gtgggactgg ctacacggag cagctgcagc aatacagcag cccaggactc 241 ggggtgaagt attacatcga cccctccacc tacgaggacc cctgtcaggc catccgagaa 301 cttgcccggg aagtcgatcc tgcttatatc acagattgag gaggtcattg ggacatggct 361 cttttggaga agtgcgccag ggccgcctgc agccacgggg acggagggag cagactgtgg 421 ccatccaggc cctgtgggcc tgggggcgcc gaaagcctgc agatgacctt cctgggccgg 481 gccgcagtgc tgggtcagtt ccagcacccc aacatcctgc ggctggaggg cgtggtcacc 541 aagagccgac ccctcatggt gctgacggag ttcatggagc ttggccccct ggacagcttc 601 ctcaggcagc gggagggcca gttcagcagc ctgcagctgg tggccatgca gcggggagtg 661 gctgctgcca tgcaggtacc tgtcccagct ttgccttcgt cccatcgcct cgctgtctgc 721 ccacagcgtt gttggtgaat agccacttgg tgtgccaagg tggcccgtct tggccaacag 781 tcctcagggg cccaagttgt ttgcttcgct gggcagcccc agaggtcatt gcacatggga 841 cagcttacga aatccaggtg atgtcctgga gccttgggat actca // LOCUS BI832028 570 bp mRNA linear EST 04-OCT-2001 DEFINITION 603076948F1 NIH_MGC_119 Homo sapiens cDNA clone IMAGE:5168891 5', mRNA sequence. ACCESSION BI832028 VERSION BI832028.1 GI:15943578 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 570) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11419 row: p column: 12 High quality sequence start: 22 High quality sequence stop: 550. FEATURES Location/Qualifiers source 1..570 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:5168891" /clone_lib="NIH_MGC_119" /tissue_type="medulla" /lab_host="DH10B" /note="Organ: brain; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source normal medulla from anonymous male age 27. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.3 kb, insert size range 0.9-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 013. Note: this is a NIH_MGC Library." BASE COUNT 136 a 131 c 193 g 110 t ORIGIN 1 ccaggctgta cgcccgagta cgggatgtca gtcctggcga agcgtctctt ctaggttcag 61 actgctttct acttgttgtg tctcgaatgg cagaaaaagg ggtggctgtt ggaggaaggg 121 aggagagtaa atgaagagaa agaactggaa taaccccttg cagaaaaaaa aaaaaaaggg 181 aagcaggctt agctgtacac cctgagtctt gcaaaagctg cagccccacc caggtagcag 241 ggtggtggct ggggccgatg gtggacgccc tgaagatgtc ccatggctac tgaaggggct 301 gcccagttag ggaacagagt ggcgggcatg gtgtgtagcc tatgggtgct gctcctggtg 361 tcttcagttc tggctctgga agaggtattg gctggacacc accggagaga catctgagat 421 gggctggctc acctacccac caggggagtg ggacgaggtg agtgtctgga cgaccagcga 481 cgcctgactc ggaccttaga ggcaatgtca tgtggcaggg gcccctccag gcaccgggca 541 ggaccaatgg gtcgcagaca acactattgg // LOCUS BI828058 570 bp mRNA linear EST 04-OCT-2001 DEFINITION 603075309F1 NIH_MGC_119 Homo sapiens cDNA clone IMAGE:5167011 5', mRNA sequence. ACCESSION BI828058 VERSION BI828058.1 GI:15939608 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 570) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11415 row: b column: 04 High quality sequence stop: 551. FEATURES Location/Qualifiers source 1..570 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:5167011" /clone_lib="NIH_MGC_119" /tissue_type="medulla" /lab_host="DH10B" /note="Organ: brain; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source normal medulla from anonymous male age 27. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.3 kb, insert size range 0.9-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 013. Note: this is a NIH_MGC Library." BASE COUNT 131 a 155 c 184 g 100 t ORIGIN 1 cgggaccagc ttggcgacgg cgatctcgac gcgggcccca ggatctcccg gcgcccgagg 61 tctggagcag cccctgccgc cagctgtcag tgtccacccc ggaatcccag gactctcggc 121 gccgaacggg acccgggccg ggtgcaactg gggtccccgg actggagaag acgcgggtgg 181 caccgtgcga gctccaggag ccccgggtcc actgcgaggc ctcggggggc gcagacctgc 241 agagactgct ggccaacggg aagaaataaa gggattatag tccacccaat tcacagactt 301 ctgagactca gacacgagga gagatagaga accgccaatc tctagatcaa caagcaaagg 361 aggtgccaag cctgttttgt cttcattgtg acacttggag tctagatgct gggaagtcca 421 agatcagggt gccggcatgg tcagttcctg gcgaagcctc tcttctaggt ttcagactgc 481 cctcttcttt gttgtgtcct cgaatggcac gaaaaagggg tggctgttgg aggaagggag 541 gagagtaaat gaagagaaag aactgcaata // LOCUS BI825061 760 bp mRNA linear EST 04-OCT-2001 DEFINITION 603039634F1 NIH_MGC_115 Homo sapiens cDNA clone IMAGE:5180481 5', mRNA sequence. ACCESSION BI825061 VERSION BI825061.1 GI:15936611 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 760) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11450 row: c column: 10 High quality sequence start: 25 High quality sequence stop: 632. FEATURES Location/Qualifiers source 1..760 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:5180481" /clone_lib="NIH_MGC_115" /lab_host="DH10B" /note="Organ: pooled brain, lung, testis; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source anonymous pool of 6 male brains, age range 23-27; 1 male lung, age 27; and 1 male testis, age 69. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.8 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 021. Note: this is a NIH_MGC Library." BASE COUNT 129 a 243 c 253 g 135 t ORIGIN 1 ggcggtaccg gccggaaggt cttaggatct ctgcagtcgt gggctgtggg accccacggg 61 gctgggcagc gggctggact gcaactgaac gtcaaagagc ggagcttagg gcctctcacc 121 caacgcggct tctacgtggc cttccaggac acgggggcct gcctggccct ggtcgctgtc 181 aggctcttct cctacacctg ccctgccgtg ctccgatcct ttgcttcctt tccagagacg 241 caggccagtg gggctggggg ggcctccctg gtggcagctg tgggcacctg tgtggctcat 301 gcagagccag aggaggatgg agtagggggc caggcaggag gcagcccccc caggctgcac 361 tgcaacgggg agggcaagtg gatggtagct gtcgggggct gccgctgcca gcctggatac 421 caaccagcac gaggagacaa ggcctgccaa gcctgaccac gggggctcta taagtcttct 481 gctgggaatg ctccctgctc accatgccct gaccgcagtc acgctcccaa cccagcagtc 541 cccgtttgcc cctgcctgga gggcttctac cgggccagtt ccgacccacc agaggccccc 601 tgcactggtc ctccatcggc tccccaggag cttaggttag acggtgcaag ggctcaagca 661 ctcattgcta cacctggcgc ctgcctcggg agctggaggg tcgaggggac ctgctcttcc 721 atgtagtgtg caaggagtgt gaaggaccgc caaggaacac // LOCUS BI761660 779 bp mRNA linear EST 25-SEP-2001 DEFINITION 603046390F1 NIH_MGC_116 Homo sapiens cDNA clone IMAGE:5186638 5', mRNA sequence. ACCESSION BI761660 VERSION BI761660.1 GI:15753238 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 779) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11466 row: c column: 23 High quality sequence stop: 769. FEATURES Location/Qualifiers source 1..779 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:5186638" /clone_lib="NIH_MGC_116" /lab_host="DH10B" /note="Organ: pooled colon, kidney, stomach; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source anonymous pool of 3 colons, age 26 yo male, 49 yo female, 71 yo male colon; 46 yo male kidney, and pool of 2 stomachs, 62 yo male and 70 yo female. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 023. Note: this is a NIH_MGC Library." BASE COUNT 175 a 194 c 265 g 144 t 1 others ORIGIN 1 caagatcagg gtgccggcat ggtcagttcc tggcgaagcc tctcttctag gtttcagact 61 gccctcttct ttgtgtgtcc tcgaatggca gaaaaagggg tggctgttgg aggaagggag 121 gagagtaaat gaagagaaag aactggaata accccttgca gaaaaaaaaa aaaagggaag 181 caagcttagc tgtacaccct gagtcttgca aaagctgcag ccccacccag gagcagggtg 241 gtggctgggg cgatggtgga cgccctgaag atgtcccatg gctactgaag gggctgccca 301 gttagggaac agagtggcgg gcatggtgtg tagcctatgg gtgctgctcc tggtgtcttc 361 agttctggct ctggaagagg tattgctgga caccaccgga gagacatctg agatggctgg 421 ctcacctacc caccaggggg gtgggacgag gtgagtgtct ggacgaccag ngacgcctga 481 ctcggacctt tgaggcatgt catgtggcag gggcccctca aggcacgggc aaggacaatg 541 ggtggcagac acacttggtg aaccggcgcg gggcccaaag ggccgcacaa ttcgattcca 601 cttctcggtg cgggcatgct ccagcctggg gtgtgacgcg tgggcacctg ccgggaggac 661 cttcaccctt taatacccgt caggctgagg agcccgaaag ccctgacagc gttttcctcc 721 gtgggcacct caaagcgcgt ggaaccaaga agacgcaggc cagtggggca tggaggggc // LOCUS BI756432 747 bp mRNA linear EST 25-SEP-2001 DEFINITION 603029243F1 NIH_MGC_114 Homo sapiens cDNA clone IMAGE:5199370 5', mRNA sequence. ACCESSION BI756432 VERSION BI756432.1 GI:15748010 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 747) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11499 row: f column: 11 High quality sequence stop: 382. FEATURES Location/Qualifiers source 1..747 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:5199370" /clone_lib="NIH_MGC_114" /lab_host="DH10B" /note="Organ: brain; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source anonymous pool of 6 male brains, age range 23-27 yo. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.5 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 019. Note: this is a NIH_MGC Library." BASE COUNT 212 a 232 c 181 g 122 t ORIGIN 1 ggacaacttc tccaaggttt ggcctctgta ccttcaggtg atgtggctca gctcagccta 61 gaagacctgc ctgccctggg catcaccctg gctggccacc agaagaagct gctgcaccac 121 atccagctcc ttcagcaaca cctgaggcag cagggctcag tggaggtctg agaatgacga 181 tacccgtgac tcagccctgg acactggtcc gagaagggac atgtgggacg tgagccgggc 241 tccaacagcc tctgtgagag atgccccaca ccaaacccaa ccctcccgat ggctgcattc 301 cctggtcctc cgcctctcca ccagccccct cctcattaaa gggaaagaac ggaaaacaaa 361 aaaacaaaaa aaaaaaaaaa aaaacaaaga aaaaacaaaa aaacaacaac aaatcaaata 421 gaagatgact gagagaacgc gtcgtcgctc ccccagctac aacgaccgca ctctttttgc 481 tggtgcccca gtgtagatgc cccctctgtc aatatagcag aggcagtcca ctaacattac 541 aactgagaat gcgggctcgg tcaacagcga ctggtcacac acaaagcagc agtcgtgccg 601 tagtaccaaa ggcacgccct gctcacgaga aagcgcgcgt gtgctcgggc cacacttact 661 ctctgtacgg ctggtgggcg caatcagctt ggtgggcggc acatgaccaa ctcggcgtag 721 gcaccccgcc cacgcggagc aatacac // LOCUS BI519782 696 bp mRNA linear EST 29-AUG-2001 DEFINITION 603062059T1 NIH_MGC_118 Homo sapiens cDNA clone IMAGE:5211255 3', mRNA sequence. ACCESSION BI519782 VERSION BI519782.1 GI:15344561 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 696) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11530 row: e column: 16 High quality sequence start: 9 High quality sequence stop: 542. FEATURES Location/Qualifiers source 1..696 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:5211255" /clone_lib="NIH_MGC_118" /tissue_type="leukocyte" /lab_host="DH10B" /note="Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source leukocytes from anonymous pool of non-activated adult donors. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7 kb, insert size range 1.2-3.3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 027. Note: this is a NIH_MGC Library." BASE COUNT 171 a 190 c 194 g 141 t ORIGIN 1 ttacggcagg gttgggtttg gtgtggggca tctctcacag aggctgttgg agcccggctc 61 acgtcccaca tgtcccttct cggaccagat gtccagggct gagtcacggg tatcgtcatt 121 ctcagacctc cactgagccc tgctgcctca ggtgttgctg aaggagctgg atgtggtgca 181 gcagcttctt ctggtggcca gccaggggtg atgcccaggg caggcaggtc ttctaggctg 241 agctgagcca catcactgaa ggtacagagg ccaaacttgg agaagttgtc ctggtagcac 301 tccagtccaa tggctgaaag ccaggcctgg ggtgagtcca gacaaggaaa gtccaggacc 361 acagggcgtc agaagggtcc tgggcaaggc ctttcaccct gggatccccg acagcctgac 421 agggtatctg gcttgcggat cactcttgtc acaatgcaga ccaccagctg gtcaacaatg 481 aggcccgcac ggagcacggt ccattctgac caggatgtcc aacataaagt agatgtacat 541 ccaggaagga cagacctgga aggcaagggg cagaccggaa ctccaatgct ctatatgcat 601 tatagtacat cctgactcac atcatgtccc agtaaaagac gttactcaca ataactcatc 661 aacttaccca catgagtaat ccgaaaactc cagaca // LOCUS BI518694 898 bp mRNA linear EST 29-AUG-2001 DEFINITION 603062059F1 NIH_MGC_118 Homo sapiens cDNA clone IMAGE:5211255 5', mRNA sequence. ACCESSION BI518694 VERSION BI518694.1 GI:15343486 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 898) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Life Technologies, Inc. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM11530 row: e column: 16 High quality sequence stop: 657. FEATURES Location/Qualifiers source 1..898 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:5211255" /clone_lib="NIH_MGC_118" /tissue_type="leukocyte" /lab_host="DH10B" /note="Vector: pCMV-SPORT6; Site_1: NotI; Site_2: EcoRV (destroyed); RNA source leukocytes from anonymous pool of non-activated adult donors. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7 kb, insert size range 1.2-3.3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 027. Note: this is a NIH_MGC Library." BASE COUNT 168 a 282 c 294 g 154 t ORIGIN 1 ggctcaccta cccaccaggg ggtgggacga ggtgagtgtt ctggacgacc agcgacgcct 61 gactcggacc tttgaggcat gtcatgtggc aggggcccct ccaggcaccg ggcaggacaa 121 ttggttgcag acacactttg tggagcggcg cggggcccag agggcgcaca ttcgactcca 181 cttctctgtg cgggcatgct ccagcctggg tgtgagcggc ggcacctgcc gggagacctt 241 caccctttac taccgtcagg ctgaggagcc cgacagccct gacagcgttt cctcctggca 301 cctcaaacgc tggaccaagg tggacacaat tgcagcagac gagagctttc cctcctcctc 361 ctcctcctcc tcctcttctt cctctgcagc gtgggctgtg ggaccccacg gggctgggca 421 gcgggctgga ctgcaactga acgtcaaaga gcggagcttt gggcctctca cccaacgcgg 481 cttctacgtg gccttccagg acacgggggc ctgcctggcc ctggtcgctg tcaggctctt 541 ctcctacacc tgccctgccg tgctccgatc ctttgcttcc tttccagaga cgcaggccag 601 tggggctggg ggggcctccc tggtggcagc tgtgggcacc atgtgtggct catgcagaag 661 ccagacggag gatgcagtag gggggcaggc aggaggcaga cccccaggct gcactgcacg 721 ggggagggca agtggatggt agctagcagg gggctggccg tgaccagctg gataccaaac 781 agaacgagga gacaacggct gccaaagctg aacaccgggg ctccataaag cttctggcgg 841 gaatgctcgc aggcaacaat ggccctgacc gcagagcagg agccaaccag aaagaccc // LOCUS BG759431 850 bp mRNA linear EST 15-MAY-2001 DEFINITION 602711973F1 NIH_MGC_48 Homo sapiens cDNA clone IMAGE:4852123 5', mRNA sequence. ACCESSION BG759431 VERSION BG759431.1 GI:14070084 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 850) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Louis M. Staudt, M.D., Ph.D. cDNA Library Preparation: Ling Hong/Rubin Laboratory cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLCM1696 row: a column: 20 High quality sequence stop: 676. FEATURES Location/Qualifiers source 1..850 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:4852123" /clone_lib="NIH_MGC_48" /tissue_type="primary B-cells from tonsils (cell line)" /lab_host="DH10B (phage-resistant)" /note="Organ: B-cells; Vector: pOTB7; Site_1: XhoI; Site_2: EcoRI; cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library." BASE COUNT 177 a 229 c 250 g 194 t ORIGIN 1 ggccagttca gcagcctgca gctggtggcc atgcagcggg gagtggctgc tgccatgcag 61 tacctgtcca gctttgcctt cgtccatcgc tcgctgtctg cccacagcgt gctggtgaat 121 agccacttgg tgtgcaaggt ggcccgtctt ggccacagtc ctcagggccc aagttgtttg 181 cttcgctggg cagccccaga ggtcattgca catggaaagc atacaacatc cagtgatgtc 241 tggagctttg ggatactcat gtgggaagtg atgagttatg gagaacggct tactgggaca 301 tgagtgagca ggaggtacta aatgcaatag agcaggagtt ccggctgccc cgctccaggc 361 tgtcctcctg gattacatct acttatgttg gacacttggc agaaggaccg tgcccggcgg 421 ctcattttga ccagctggtg gctgcatttg acaagatgat ccgcaagcca gataccctgc 481 aggctggcgg gaccagggaa aagggcttcc cagggccctt ctgacccctg tgtgcctgga 541 ctttccttgt ctgactcacc ccaggcctgg ctttcagcat ttgactgagt gtaccaggac 601 aacttctcca agtttggtct ctgtacttcc agtgatgtgg tcagtcagct agaagactgc 661 ctgcctggga tcacctgggt ggccaccaga agaagttgtg accaaatcca gtcctttcag 721 aacaaccttg aagcagcggc tccagtgagg gcctgagaat gacgataccc ggaattcagc 781 ctggacctgt cccgcggaag ggcttgttgg acggagccgg gtcaaaagct ctgtgtgaaa 841 tgccaacaac // LOCUS BG289703 776 bp mRNA linear EST 21-FEB-2001 DEFINITION 602384683F1 NIH_MGC_93 Homo sapiens cDNA clone IMAGE:4513557 5', mRNA sequence. ACCESSION BG289703 VERSION BG289703.1 GI:13045757 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 776) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: ATCC cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM10400 row: f column: 22 High quality sequence stop: 718. FEATURES Location/Qualifiers source 1..776 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:4513557" /clone_lib="NIH_MGC_93" /tissue_type="transitional cell papilloma, cell line" /lab_host="DH10B (phage-resistant)" /note="Organ: bladder; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: SalI; Cloned unidirectionally; oligo-dT primed. Average insert size 1.7 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library." BASE COUNT 181 a 224 c 214 g 156 t 1 others ORIGIN 1 gcagccccag aggtcattgc acatggaaag catacaacat ccagtgatgt ctggagcttt 61 gggatactca tgtgggaagt gatgagttat ggagaacggc cttactggga catgagtgag 121 caggaggtac taaatgcaat agagcaggag ttccggctgc ccccgcctcc aggctgtcct 181 cctggattac atctacttat gttggacact tggcagaagg accgtgcccg gcggcctcat 241 tttgaccagc tggtggctgc atttgacaag atgatccgca agccagatac cctgcaggct 301 ggcggggacc caggggaaag gccttcccag gcccttctga cccctgtggc cctggacttt 361 ccttgtctgg actcacccca ggcctggctt tcagccattg gactggagtg ctaccaggac 421 aacttctcca agtttggcct ctgtaccttc agtgatgtgg ctcagctcag cctagaagac 481 ctgcctgctc tgggcatcac cctggctggc caccagaaga agctgctgca ccacatccag 541 ctccttcagc aacacctgag gcagcagggc tcagtggagg tctgagaatg acgatacccg 601 tgactcagcc ctggacactg gtccgagaag ggacatgtgg gacgtgagcc gggctccaac 661 agnctctgtg ggaagatgcc caaacaaaac caacctcgat ggtgattccc tggtcctcgg 721 tctcacaggc cctctcataa gggcagaagg caaaataaca aacaccgaca cacaca // LOCUS BG287546 883 bp mRNA linear EST 21-FEB-2001 DEFINITION 602384558F1 NIH_MGC_93 Homo sapiens cDNA clone IMAGE:4513479 5', mRNA sequence. ACCESSION BG287546 VERSION BG287546.1 GI:13041486 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 883) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: ATCC cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM10400 row: c column: 16 High quality sequence stop: 603. FEATURES Location/Qualifiers source 1..883 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:4513479" /clone_lib="NIH_MGC_93" /tissue_type="transitional cell papilloma, cell line" /lab_host="DH10B (phage-resistant)" /note="Organ: bladder; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: SalI; Cloned unidirectionally; oligo-dT primed. Average insert size 1.7 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library." BASE COUNT 175 a 283 c 284 g 140 t 1 others ORIGIN 1 gcggcggcac ctgccgggag accttcaccc tttactaccg tcaggctgag gagcccgaca 61 gccctgacag cgtttcctcc tggcacctca aacgctggac caaggtggac acaattgcag 121 cagacgagag ctttccctcc tcctcctcct cctcctcctc ctcctcttct tcctctgcag 181 cgtgggctgt gggaccccac ggggctgggc agcgggctgg actgcaactg aacgtcaaag 241 agcggagctt tgggcctctc acccaacgcg gcttctacgt ggccttccag gacacggggg 301 cctgcctggc ctggtcgctg tcaggctctt ctcctacacc tgccctgccg tgctccgatc 361 ctttgcttcc tttccagaga cgcaggccag tggggctggg ggggcctccc tggtggcagc 421 tgtgggcacc tgtgtggctc atgcagagcc agaggaggat ggagtagggg gccaggcagg 481 aggcagcccc ccaggctgca actgcaacgg ggagggcaag tggatggtag ctgtcggggg 541 ctgccgactg caggcctgaa tacaacagca cgaggagaca aggctgccaa gcctgcccac 601 gggggctcct ataagccttc ctggctggga aatggtcccc tggtccaccc aatggcgctg 661 ccccgaagtc acgcctcccg aacaccaaga agaccccccg gttggcccct gctggaaggg 721 acactaaccg gggcaagtac gaccaacaga agggcccctg gcagggggcc tacatgaaga 781 ccccaaggac ctgagtagga gggcaaggac actcatggtc atggcgcgcc cgagagggga 841 agagggagga caacgctcgg agaagaagga gagaacaaca gcn // LOCUS BF238114 780 bp mRNA linear EST 14-NOV-2000 DEFINITION 601811834F1 NIH_MGC_48 Homo sapiens cDNA clone IMAGE:4054617 5', mRNA sequence. ACCESSION BF238114 VERSION BF238114.1 GI:11152033 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 780) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Louis M. Staudt, M.D., Ph.D. cDNA Library Preparation: Ling Hong/Rubin Laboratory cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLCM895 row: d column: 10 High quality sequence stop: 696. FEATURES Location/Qualifiers source 1..780 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:4054617" /clone_lib="NIH_MGC_48" /tissue_type="primary B-cells from tonsils (cell line)" /lab_host="DH10B (phage-resistant)" /note="Organ: B-cells; Vector: pOTB7; Site_1: XhoI; Site_2: EcoRI; cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library." BASE COUNT 129 a 236 c 259 g 156 t ORIGIN 1 ggtcttccca gcttccggaa agactctcct gggtgatcgg ctccatcctg ggggctttgg 61 ccttcctcct gctggcagcc atcaccgtgc tggcggtcgt cttccagcgg aagcggcgtg 121 ggactggcta cacggagcag ctgcagcaat acagcagccc aggactcggg gtgaagtatt 181 acatcgaccc ctccacctac gaggacccct gtcaggccat ccgagaactt gcccgggaag 241 tcgatcctgc ttatatcaag attgaggagg tcattgggac aggctctttg gagaagtgcg 301 ccaggccgct gcagccacgg ggacggaggg agcagactgt ggccatccag gccctgtggg 361 ccgggggcgc cgaaagcctg cagatgacct tcctgggccg ggccgcagtg ctgggtcagt 421 tccagcaccc caacatcctg cggctggagg gcgtggtcac caagagccga cccctcatgg 481 tgctgacgga gttcatggag cttggcccct ggacagcttc ctcaggcagc gggagggcca 541 gttcagcagc ctgcagctgg tggccatgca gcggggagtg gctgctgcca tgcagtacct 601 gtccagcttt gccttcgtcc atcgctcgct gtctgcccac aggtgctggt gaatagccac 661 ttggtgtgca aggtggccgt cttggcacag tcctcagggc ccagtgttgt tccgctgggc 721 agcccagagg tcttgcctgg aaagctacac tccagtgtgt ctgggcttgg gtctctgggg // LOCUS BF222567 153 bp mRNA linear EST 09-NOV-2000 DEFINITION 7p54f08.x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE:3649694 3', mRNA sequence. ACCESSION BF222567 VERSION BF222567.1 GI:11129744 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 153) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Michael J. Brownstein, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL, send email to: info@image.llnl.gov. FEATURES Location/Qualifiers source 1..153 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:3649694" /clone_lib="NCI_CGAP_Pr28" /sex="male" /dev_stage="adult" /lab_host="DH10B" /note="Organ: prostate; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Plasmid DNA from the normalized library NCI_CGAP_Pr22 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 985608-986759, 1101192-1101959, and 1217928-1220615). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 25 a 33 c 52 g 43 t ORIGIN 1 tttttttttt tgcaaattcc cttctttccc tttaatgagg agggggctgg tggagaggcg 61 gaggaccagg gaatgcagcc atcgggaggg ttgggtttgg tgtggggcat ctctcacaga 121 ggctgttgga gcccggctca cgtcccacat gtc // LOCUS BF034648 876 bp mRNA linear EST 20-OCT-2000 DEFINITION 601455086F1 NIH_MGC_66 Homo sapiens cDNA clone IMAGE:3858956 5', mRNA sequence. ACCESSION BF034648 VERSION BF034648.1 GI:10742360 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 876) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: DCTD/DTP cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM9591 row: k column: 21 High quality sequence stop: 679. FEATURES Location/Qualifiers source 1..876 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:3858956" /clone_lib="NIH_MGC_66" /tissue_type="adenocarcinoma" /lab_host="DH10B (phage-resistant)" /note="Organ: ovary; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: SalI; Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.8 kb. Library constructed by Life Technologies. " BASE COUNT 179 a 289 c 263 g 145 t ORIGIN 1 gcaggctgtt aatggggtgt ctgagctcag ccctgaccct cctcaggctg cagccatcaa 61 tgtcagcacc agccatgaag tgccctctgc tgtccctgtg gttcaccagg tgagccgggc 121 atccaacagc atcacggtgt cctggccgca gcccgaccag accaatggga acatcctgga 181 ctatcagctc cgctactatg accaggcaga agacgaatcc cactccttca ccctgaccag 241 cgagaccaac actgccaccg tgacacagct gagccctggc cacatctatg gtttccaggt 301 gcgggcccgg actgctgccg gccacggccc tacgggggca aagtctattt ccagacactt 361 cctcaagggg agctgtcttc ccagcttccg gaaagactct ccttggtgat cggctccatc 421 ctgggggctt tggccttcct cctgctggca gccatcaccg tgctggcggt cgtcttccag 481 cggaagcggc gtgggactgg ctacacggag cagctgcagc aatacagcag ccaggactcg 541 gggtgaagta ttacatcgac cctccaccta cgaggaccct gtcaggccat ccgagaactt 601 gcccgggaag tcgatcctgc ttatatcaac gatgaggagg ccattgggac aggcctcttt 661 ggagaagtgc gccaggccgg ctggcagcca ccgggcacga gggagccaca attgggggca 721 tccaaggccg tggggccggg gcgccgaaag cccgaaatga cctccttggg ccggccacag 781 ctgggcacgt ccacgacccc acatctgggg ggagggcggg ccccaaaaca caccccacgg 841 gtgccggaga tgaggggcgc gcgacaagac ccaacg // LOCUS BE909661 454 bp mRNA linear EST 20-OCT-2000 DEFINITION 601501669F1 NIH_MGC_70 Homo sapiens cDNA clone IMAGE:3903473 5', mRNA sequence. ACCESSION BE909661 VERSION BE909661.1 GI:10405480 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 454) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: ATCC cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLAM9707 row: j column: 18 High quality sequence stop: 454. FEATURES Location/Qualifiers source 1..454 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:3903473" /clone_lib="NIH_MGC_70" /tissue_type="epithelioid carcinoma" /lab_host="DH10B (phage-resistant)" /note="Organ: pancreas; Vector: pCMV-SPORT6; Site_1: NotI; Site_2: SalI; Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Library constructed by Life Technologies." BASE COUNT 111 a 134 c 146 g 63 t ORIGIN 1 acccagcttg gcgaacggcg atctcgacgc gggcccccag gatctcccgg cgccccacct 61 ctggagcagc ccctgccgca gcgtcaggtc caccccggaa tcccaggact ctcggcgccg 121 aacggacccg ggccgggtgc aacggggtcc ccggactgga gaagacgcgg gtggcaccgt 181 gcgagatcca ggagccccgg gtccactgcg aggcctcggg gggcgcagac ctgcagagac 241 tgcggccaac gggaagaaat aaagggatta tagtccaccc aattcacaga cttctgagac 301 tcagacacga ggagagatag agaaccgcca atctctagat caacaagcaa aggaggtgcc 361 aagcctgttt gtcttcattg tgacactgga gggtggctgt tggaggaagg gaggagagta 421 catgacagag aaagaactgg aataacccct tgca // LOCUS BE856104 137 bp mRNA linear EST 29-SEP-2000 DEFINITION 7f88a02.x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE:3304010 3', mRNA sequence. ACCESSION BE856104 VERSION BE856104.1 GI:10368803 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 137) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Michael J. Brownstein, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL, send email to: info@image.llnl.gov Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..137 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:3304010" /clone_lib="NCI_CGAP_Pr28" /sex="male" /dev_stage="adult" /lab_host="DH10B" /note="Organ: prostate; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Plasmid DNA from the normalized library NCI_CGAP_Pr22 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 985608-986759, 1101192-1101959, and 1217928-1220615). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 22 a 32 c 51 g 32 t ORIGIN 1 ttcccttctt tccctttaat gaggaggggg ctggtggaga ggcggaggac cagggaatgc 61 agccatcggg agggttgggt ttggtgtggg gcatctctca cagaggctgt tggagcccgg 121 ctcacgtccc acatgtc // LOCUS BE736188 972 bp mRNA linear EST 15-SEP-2000 DEFINITION 601307287F1 NIH_MGC_39 Homo sapiens cDNA clone IMAGE:3641685 5', mRNA sequence. ACCESSION BE736188 VERSION BE736188.1 GI:10150180 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 972) AUTHORS NIH-MGC http://mgc.nci.nih.gov/. TITLE National Institutes of Health, Mammalian Gene Collection (MGC) JOURNAL Unpublished (1999) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: ATCC cDNA Library Preparation: Ling Hong/Rubin Laboratory cDNA Library Arrayed by: The I.M.A.G.E. Consortium (LLNL) DNA Sequencing by: Incyte Genomics, Inc. Clone distribution: MGC clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: http://image.llnl.gov Plate: LLCM347 row: n column: 22 High quality sequence stop: 660. FEATURES Location/Qualifiers source 1..972 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:3641685" /clone_lib="NIH_MGC_39" /tissue_type="adenocarcinoma" /lab_host="DH10B (phage-resistant)" /note="Organ: pancreas; Vector: pOTB7; Site_1: XhoI; Site_2: EcoRI; cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies)." BASE COUNT 211 a 254 c 302 g 204 t 1 others ORIGIN 1 tagtgggggg actccgggca cacgtaccct acatcttaga ggtgcaggct gttaatgggg 61 tgtctgagct cagccctgac cctcctcagg ctgcagccat caatgtcagc accagccatg 121 aagtgccctc tgctgtccct gtggtgcacc aggtgagccg ggcatccaac agcatcacgg 181 tgtcctggcc gcagcccgac cagaccaatg ggaacatcct ggactatcag ctccgctact 241 atgaccaggc agaagacgaa tcccactcct tcaccctgac cagcgagaac aacactgcca 301 ccgtgacaca gctgagccct ggccacatct atggtttcca ggtgcgggcc cggactgtgc 361 cggccacggc ccctacgggg gcaaagtcta tttccagaca cttcctcaag gggagctgtc 421 ttcccagctt ccggaaagac tctccttggt gatcggctcc atcctggggg ctttggcttc 481 ctcctgctgg cagccatcac cgtgctggcg gtcgtcttcc agcggaagcg gcgtgggact 541 ggtacacgga gcagtgcagc aatacagcag ccaggactcg gggtgaagta ttacatcgac 601 cctccaacta gaggaccctg tagggcatcc gagaacttgc cgggaagtcg atcctgttat 661 attcagattg aggaggtcat tgcgacaggg tcttttggag acgtgggcca aggcgctgag 721 gcaggggacg ggggaagcag atgtggcatc caagctgtgg gcggggggcg aaagcttgaa 781 ataacttctg ggcggggatg tgggtagttc acgacccaaa tctgcgtggg gggtggtgca 841 aaaagggctc tgtgtgggaa taagagggcc ggaaatatga aggggaaact gtggttaggg 901 ggtgtaatca tttcaatgca taagaaatag gggctaccat ttgacatatt attgtatttt 961 gttgttcttg gn // LOCUS AV645352 662 bp mRNA linear EST 15-JAN-2002 DEFINITION AV645352 GLA Homo sapiens cDNA clone GLAAAD05 3', mRNA sequence. ACCESSION AV645352 VERSION AV645352.1 GI:9866366 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 662) AUTHORS Xu,X., Huang,J., Xu,Z., Qian,B., Zhu,Z., Yan,Q., Cai,T., Zhang,X., Xiao,H., Qu,J., Liu,F., Huang,Q., Cheng,Z., Li,N., Du,J., Hu,W., Shen,K., Lu,G., Fu,G., Zhong,M., Xu,S., Gu,W., Huang,W., Zhao,X., Hu,G., Gu,J., Chen,Z. and Han,Z. TITLE Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver JOURNAL Proc. Natl. Acad. Sci. U.S.A. 98 (26), 15089-15094 (2001) MEDLINE 21625106 COMMENT Contact: Zeguang Han Chinese National Human Genome Center at Shanghai 351 Guo Shoujing Road, Zhangjiang Hi-Tech Park, Pudong, Shanghai 201203, P. R. China Tel: 86-21-50801919(ex.45) Fax: 86-21-50801922 Email: hanzg@chgc.sh.cn This clone is available at CHGC in Shanghai. FEATURES Location/Qualifiers source 1..662 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="GLAAAD05" /clone_lib="GLA" /tissue_type="corresponding non cancerous liver tissue" /dev_stage="Adult" /lab_host="SOLR" /note="Vector: pBluescript sk(-); Site_1: EcoRI; Site_2: XhoI" BASE COUNT 114 a 203 c 186 g 157 t 2 others ORIGIN 1 gttctttctc ttcatttact ctcctccctt cctccaacag ccaccccttt ttctgccatt 61 cgaggacaca acaaagaaga gggcagtctg aaacctagaa gagaggcttc gccaggaact 121 gaccatgccg gcaccctgat cttggacttc ccagcatcta gactccagtg tcacaatgaa 181 gacaaacagg cttggcacct cctttgcttg ttgatctaga gattggcggt tctctatctc 241 tcctcgtgtc tgagtctcag aagtctgtga attgggtgga ctataatccc tntatttctt 301 cccgttggcc gcagtctctg caggtctgcg ccccccgagg cctcgcagtg gaccccgggc 361 tctggagctc gcacggtgcc acccgcgttt tctcagtccg ggaccccgtg cacccggccc 421 ggtccgtcgg cgcgagagtc ctgggattcc gggtggacct gaggcttgcg cagggctgct 481 ccagagtggg cgccggagat cttggggccg cgtcagatcg ccgtcgcaag ctggtccggt 541 cccgccgccg tgtgaaagcc gngttgtccc aatcttgagc cgggatcggc ggctaaacgc 601 cgaacgcgtg actcagtttg ttcctaaagg ggtaattcgc ttgggatcgt tcggatgcgg 661 ta // LOCUS BE044150 376 bp mRNA linear EST 08-JUN-2000 DEFINITION ho39a02.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE:3039722 3' similar to TR:O15197 O15197 EPH-FAMILY PROTEIN. ;, mRNA sequence. ACCESSION BE044150 VERSION BE044150.1 GI:8361203 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 376) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Trace considered overall poor quality Seq primer: -40UP from Gibco High quality sequence stop: 1. FEATURES Location/Qualifiers source 1..376 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:3039722" /clone_lib="Soares_NFL_T_GBC_S1" /lab_host="DH10B" /note="Organ: pooled; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid DNA from three normalized libraries (fetal lung NbHL19W, testis NHT, and B-cell NCI_CGAP_GCB1) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 297480-302087, 682632-687239, 726408-728711, and 729096-731399. Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 79 a 113 c 108 g 76 t ORIGIN 1 cctccactga cccctgctgc ctcaggtgtt gctgaaggac ctggatgtgg tgcaccagct 61 tcttctggtg gccacccagg gtgatgccca gggcaggcag gtcttctagg ctgagctgag 121 ccacatcact gaaggtacaa aggccaaact tggaaaagtt gtcctggtag cactccagtc 181 caatggctga aagccaggcc tggggtgagt ccagacaagg aaagtccaag gccacagggg 241 tcaaaagggc ctgggaaggc ctttcccctg ggtccccgcc caccctcagg gtatcttgct 301 tgcggatcat tttgtcaaat gcagccacca gcctgtcaaa atgaggcccc cgggcccggt 361 cccttttcca agtgtc // LOCUS AW965343 465 bp mRNA linear EST 01-JUN-2000 DEFINITION EST377461 MAGE resequences, MAGI Homo sapiens cDNA, mRNA sequence. ACCESSION AW965343 VERSION AW965343.1 GI:8155224 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 465) AUTHORS Hegde,P., Qi,R., Abernathy,K., Dharap,S., Gaspard,R., Gay,C., Holt ,I.E., Saeed,A.I., Sharov,V., Lee,N.H., Yeatman,T.J. and Quackenbush,J. TITLE Assessment of gene expression patterns in a model of colon tumor metastasis using a 19,200 element cDNA microarray JOURNAL Unpublished (2000) COMMENT Contact: John Quackenbush The Institute for Genomic Research 9712 Medical Center Dr., Rockville, MD 20850, USA Tel: 301 838 3528 Fax: 301 838 0208 Email: johnq@tigr.org Plate: 214 Seq primer: Reverse. FEATURES Location/Qualifiers source 1..465 /organism="Homo sapiens" /db_xref="taxon:9606" /clone_lib="MAGE resequences, MAGI" /note="Vector: pBluescriptSKm" BASE COUNT 103 a 128 c 128 g 106 t ORIGIN 1 tggctgggca gccccagagg tcattgcaca tggaaagcat acaacatcca gcgatgtctg 61 gagctttggg atactcatgt gggaagtgat gagttatgga gaacggcctt actgggacat 121 gagtgagcag gaggtactaa atgcaatata gcaggagttc cggatgcccc cgcctccagg 181 ctgccctcct ggataacatc tacttatgtt tgacacttgg cagaaggacc gtgcccggcg 241 gcctcatttt gaccagctgg tggctgcatt tgacaagatg atccgcaagc catataccct 301 gcaggctggc ggagacccag gggaaaggcc ttcccaggcc cttctgaccc ctgtggccct 361 ggactttcct tgtctggact caccccaggc ctggctttca accattggac tggagtgcta 421 ccaggaacaa cttctccaag tttggcctct gtaccctcaa tgatg // LOCUS AW953335 337 bp mRNA linear EST 01-JUN-2000 DEFINITION EST365405 MAGE resequences, MAGB Homo sapiens cDNA, mRNA sequence. ACCESSION AW953335 VERSION AW953335.1 GI:8143018 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 337) AUTHORS Hegde,P., Qi,R., Abernathy,K., Dharap,S., Gaspard,R., Gay,C., Holt ,I.E., Saeed,A.I., Sharov,V., Lee,N.H., Yeatman,T.J. and Quackenbush,J. TITLE Assessment of gene expression patterns in a model of colon tumor metastasis using a 19,200 element cDNA microarray JOURNAL Unpublished (2000) COMMENT Contact: John Quackenbush The Institute for Genomic Research 9712 Medical Center Dr., Rockville, MD 20850, USA Tel: 301 838 3528 Fax: 301 838 0208 Email: johnq@tigr.org Plate: 49 Seq primer: Reverse. FEATURES Location/Qualifiers source 1..337 /organism="Homo sapiens" /db_xref="taxon:9606" /clone_lib="MAGE resequences, MAGB" /note="Vector: pBluescriptSKm" BASE COUNT 66 a 108 c 86 g 77 t ORIGIN 1 tggcacgagg gatccgcaag ccatataccc tgttggctgg cttggaccca ggggaaaggc 61 cttcccaggc ccttctgacc cctgtggccc tggactttcc ttgtctggac tcaccccagg 121 cctggctttc agccattgga ctggagtgct accaggacaa cttctccaag tttggcctct 181 gtaccttcag tgatgtggct catctcagcc taaaagacct gcctgccctg ggcatcaccc 241 tggctggcca ccagaagaag ctgctgcacc acatccagct ccttcagcat cacctgaggc 301 agtagggctc attggaggtc tgagaatgac tatactc // LOCUS AW664114 340 bp mRNA linear EST 06-APR-2000 DEFINITION hi04d03.x1 NCI_CGAP_GU1 Homo sapiens cDNA clone IMAGE:2971301 3' similar to TR:O15197 O15197 EPH-FAMILY PROTEIN. ;, mRNA sequence. ACCESSION AW664114 VERSION AW664114.1 GI:7456654 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 340) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Chris Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: Christa Prange, The I.M.A.G.E. Consortium DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: image.llnl.gov/image/html/iresources.shtml Trace considered overall poor quality Seq primer: -40UP from Gibco High quality sequence stop: 1. FEATURES Location/Qualifiers source 1..340 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:2971301" /clone_lib="NCI_CGAP_GU1" /tissue_type="2 pooled high-grade transitional cell tumors" /lab_host="DH10B" /note="Organ: genitourinary tract; Vector: pCMV-SPORT6; Site_1: SalI; Site_2: NotI; Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies." BASE COUNT 76 a 97 c 97 g 70 t ORIGIN 1 cctccactga cccctgctgc ctcaggtgtt gctgaaggag ctggatgtgg tgcaccaact 61 tcttttggtg gccagccagg gtgatgccca gggcaggcag gtcttctagg ctgaactgag 121 ccacatcact gaaggtacaa aggccaaact tggaaaagtt gtcctggtag cactccagtc 181 caatggctga aagccaggcc tggggtgagt ccaaacaagg aaattccagg gccacagggg 241 gcaaaagggc cttgaaaggc ctttcccctt ggtccccgcc cgcctgcagg gtatctggct 301 tgcggatcat cttgtcaaat gcagcccccc atctgtcaaa // LOCUS AW452645 202 bp mRNA linear EST 17-FEB-2000 DEFINITION UI-H-BI3-alu-f-08-0-UI.s1 NCI_CGAP_Sub5 Homo sapiens cDNA clone IMAGE:3068702 3', mRNA sequence. ACCESSION AW452645 VERSION AW452645.1 GI:6993421 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 202) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov The sequence contained an oligo-dT track that was present in the oligonucleotide that was used to prime the synthesis of first strand cDNA and therefore this may represent a bonafide poly A tail. cDNA Library Preparation: M.B. Soares Lab Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..202 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:3068702" /clone_lib="NCI_CGAP_Sub5" /lab_host="DH10B (Life Technologies)" /note="Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; NCI_CGAP_Sub5 is a subtracted library derived from NCI_CGAP_Sub4. The NCI_CGAP_Sub5 library had 3 million recombinants. A single-stranded DNA preparation of NCI_CGAP_Sub4 was used as a tracer in a subtractive hybridization with a driver comprising: the IMAGE pool (NCI_CGAP_Kid3 pool 1 LLAM 3334-3337, 3682-3683, 3798-3803 (IMAGE CloneIDs 1322376-1323911, 1456008-1456775,1500552-1502855); NCI_CGAP_Kid5 pool 1 LLAM 3338-3342,3722-3725, 3776-3778 (IMAGE CloneIDs 1323912-1325831, 1471368-1472903, 1492104-1493255); NCI_CGAP_Lu5 pool 1 LLAM 3575-3582, 3851-3854 (IMAGE CloneIDs 1414920-1417991,1520904-1522439 ); NCI_CGAP_GC4 pool 1 LLAM 3164-3167, 3716-3720, 3733-3735 (IMAGE CloneIDs 1257096-1258631,1469064-1470983, 1475592-1476743); NCI_CGAP_Pr22 pool 1 LLAM 2457-2459, 2758-2759, 3062-3068 (IMAGE CloneIDs 985608-986759 ,1101192-1101959, 1217928-1220615); NCI_CGAP_Co10 pool 1 LLAM 2644-2653, 2871-2872 (IMAGE CloneIDs 1057416-1061255 ,1144584-1145351). (10% of the driver population), plus a pool of 3,840 arrayed clones from NCI_CGAP_Sub1 (IMAGE CloneIDs 2708616-2710535) and NCI_CGAP_Sub2 (IMAGE CloneIDs 2710536-2712455) (10% of the driver population ), plus a pool of 11,136 clones from NCI_CGAP_Sub3 (IMAGE CloneIDs 2712456-2723591) (10% of the driver population), plus a pool of 5,472 clones from NCI_CGAP_Sub4 (IMAGE CloneIDs 2723592-2728969) (70% of the driver population). Subtraction was performed as previously described [Bonaldo , Lennon & Soares (1996): Normalization and Subtraction: Two Approaches To Facilitate Gene Discovery. Genome Research 6, 791-806. TAG_LIB=NCI_CGAP_Pr22 TAG_TISSUE=prostate TAG_SEQ=AAGTG" BASE COUNT 30 a 49 c 65 g 58 t ORIGIN 1 tttttttttt tttttttgca aattcccttc tttcccttta atgaggaggg ggctggtgga 61 gaggcggagg accagggaat gcagccatcg ggagggttgg gtttggtgtg gggcatctct 121 cacagaggct gttggagccc ggctcacgtc ccacatgtcc cttctcggac cagtgtccag 181 ggctgagtca cgcctcgtgc cg // LOCUS AW237437 234 bp mRNA linear EST 13-DEC-1999 DEFINITION xm71h07.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2689693 3', mRNA sequence. ACCESSION AW237437 VERSION AW237437.1 GI:6569826 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 234) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..234 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:2689693" /clone_lib="NCI_CGAP_Kid11" /lab_host="DH10B" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 58 a 63 c 45 g 68 t ORIGIN 1 caaggggtta ttccagttct ttctcttcat ttactctcct cccttcctcc aacagccacc 61 cctttttctg ccattcgagg acacaacaaa gaagagggca gtctgaaacc tagaagagag 121 gcttcgccag gaactgacca tgccggcacc ctgatcttgg acttcccagc atctagactg 181 tgagaaagat atttctattg tgtataagct actaggtcta ttgcactttg ttat // LOCUS AW237419 236 bp mRNA linear EST 13-DEC-1999 DEFINITION xm71f07.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE:2689669 3', mRNA sequence. ACCESSION AW237419 VERSION AW237419.1 GI:6569808 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 236) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..236 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:2689669" /clone_lib="NCI_CGAP_Kid11" /lab_host="DH10B" /note="Organ: kidney; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 58 a 62 c 46 g 70 t ORIGIN 1 tgtaaggggt tattccagtt ctttctcttc atttactctc ctcccttcct ccaacagcca 61 cccctttttc tgccattcga ggacacaaca aagaagaggg cagtctgaaa cctagaagag 121 aggcttcgcc aggaactgac catgccggca ccctgatctt ggacttccca gcatctagac 181 tgtgagaaag atatttctat tgtgtataag ctactaggtc tattgcactt tgttat // LOCUS AW074097 641 bp mRNA linear EST 13-OCT-1999 DEFINITION xb07f08.x1 NCI_CGAP_GU1 Homo sapiens cDNA clone IMAGE:2575623 3' similar to TR:O15197 O15197 EPH-FAMILY PROTEIN. ;, mRNA sequence. ACCESSION AW074097 VERSION AW074097.1 GI:6029095 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 641) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Chris Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: Christa Prange, The I.M.A.G.E. Consortium DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from Gibco High quality sequence stop: 129. FEATURES Location/Qualifiers source 1..641 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:2575623" /clone_lib="NCI_CGAP_GU1" /tissue_type="2 pooled high-grade transitional cell tumors" /lab_host="DH10B" /note="Organ: genitourinary tract; Vector: pCMV-SPORT6; Site_1: SalI; Site_2: NotI; Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies." BASE COUNT 158 a 173 c 186 g 123 t 1 others ORIGIN 1 gcaaattccc ttctttccct ttaatgagga ggggggtggt ggagaggcgg aggaccangg 61 aatgcaccca tcgggagggt tgggtttggt gtggggcatc tctcacagag gctgttggag 121 cccggctcac gtcccacatg tcccttctcg gaccagtgtc caaggctgag tcacaggtat 181 cgacattctc agacctccac tgagccctgc tgcctcaggt gttgctgaag gaactggatg 241 tggtgcaaca gcttcttctg gtggccagcc agggtgatgc ccaaggcacg caggtcttct 301 aggctgagct gacccacatc actgaaggta cagaagccaa acttggagaa attgtcctgg 361 tagcactcca atccaatggc tgaaagccag gcctggggtg agtccagaca aggaaagtcc 421 aaggccacag ggggcaaaaa ggcccgggaa agccttcacc catggtcccg ccagcctgca 481 gggaatatgg cttgccgcac atctggcata tgcagcgacc aactcgtcaa aagtaagacg 541 accggacctg ggcctctccc aatgatcaca atcatgagag ctaatgcatg acgacaacct 601 gaagcgaggg ccccagaaat ctgatctatt gcagtaaaga c // LOCUS AI923695 680 bp mRNA linear EST 08-MAR-2000 DEFINITION wn59e10.x1 NCI_CGAP_Lu19 Homo sapiens cDNA clone IMAGE:2449770 3' similar to TR:O15197 O15197 EPH-FAMILY PROTEIN. ;, mRNA sequence. ACCESSION AI923695 VERSION AI923695.1 GI:5659659 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 680) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 1164 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 460. FEATURES Location/Qualifiers source 1..680 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:2449770" /clone_lib="NCI_CGAP_Lu19" /tissue_type="squamous cell carcinoma, poorly differentiated (4 pooled tumors, including primary and metastatic)" /dev_stage="adult" /lab_host="DH10B (phage-resistant)" /note="Organ: lung; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; 1st strand cDNA was prepared from pooled lung tumor tissue, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went througb one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 134 a 187 c 209 g 148 t 2 others ORIGIN 1 ttttcccttc tttcccttta atgaggaggg ggctggtgga gaggcggagg accagggaat 61 gcagccatcg ggagggttgg gtttggtgtg gggcatctct cacagaggct gttggagccc 121 ggctcacgtc ccacatgtcc cttctcggac cagtgtccag ggctgagtca cgggtatcgt 181 cattctcaga cctccactga gccctgctgc ctcaggtgtt gctgaaggag ctggatgtgg 241 tgcagcagct tcttctggtg gccagccagg gtgatgccca gggcaggcag gtcttctagg 301 ctgagctgag ccacatcact gaaggtacag aggccaaact tggagaagtt gtcctggtag 361 cactccagtc caatggctga aagccaggcc tggggtgagt ccagacaagg aaagtccagg 421 gccacagggg tcagaagggc ctgggaaggc ctttcccctg ngtccccgcc agcctgcang 481 gtatctggct tgcggatcat cttgtcaaat gcagccacca gctggtcaaa atgaggccgg 541 cgggcacggt ccttctgccc agtgtccaac ataagtagat gtaatccaag aggacaacct 601 ggaagcctgg gccagccgaa ctcctgcttt attgcattta gttcctcctg ctcactcatg 661 tcccaatagg gccgtcctcc // LOCUS AI800066 153 bp mRNA linear EST 06-JUL-1999 DEFINITION wc15b06.x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE:2315219 3', mRNA sequence. ACCESSION AI800066 VERSION AI800066.1 GI:5365538 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 153) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Michael J. Brownstein, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..153 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:2315219" /clone_lib="NCI_CGAP_Pr28" /sex="male" /dev_stage="adult" /lab_host="DH10B" /note="Organ: prostate; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Plasmid DNA from the normalized library NCI_CGAP_Pr22 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 985608-986759, 1101192-1101959, and 1217928-1220615). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 25 a 32 c 52 g 44 t ORIGIN 1 tttttttttt tgcaaattcc tttctttccc tttaatgagg agggggctgg tggagaggcg 61 gaggaccagg gaatgcagcc atcgggaggg ttgggtttgg tgtggggcat ctctcacaga 121 ggctgttgga gcccggctca cgtcccacat gtc // LOCUS AI668856 214 bp mRNA linear EST 17-DEC-1999 DEFINITION wb82a11.x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE:2312156 3' similar to TR:O15197 O15197 EPH-FAMILY PROTEIN. ;, mRNA sequence. ACCESSION AI668856 VERSION AI668856.1 GI:4833630 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 214) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Michael J. Brownstein, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 299 Std Error: 0.00 Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..214 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:2312156" /clone_lib="NCI_CGAP_Pr28" /sex="male" /dev_stage="adult" /lab_host="DH10B" /note="Organ: prostate; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Plasmid DNA from the normalized library NCI_CGAP_Pr22 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 985608-986759, 1101192-1101959, and 1217928-1220615). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 28 a 70 c 69 g 47 t ORIGIN 1 tttttttttt tttcccttct ttccctttaa tgaggagggg gctggtggag aggcggagga 61 ccacgccctc cagccgcagg atgttggggt gctggaactg acccagcact gcggcccggc 121 ccaggaaggt catctgcagg ctttcggcgc ccccggccca cagggcctgg atggccacag 181 tctgctccct ccgtccccgt ggctgcaggc ggcc // LOCUS AI590650 503 bp mRNA linear EST 14-MAY-1999 DEFINITION tw24h10.x1 NCI_CGAP_Ov35 Homo sapiens cDNA clone IMAGE:2260675 3' similar to TR:O15197 O15197 EPH-FAMILY PROTEIN. ;, mRNA sequence. ACCESSION AI590650 VERSION AI590650.1 GI:4599698 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 503) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher A. Moskaluk, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: Life Technologies, Inc. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 701 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 119 POLYA=No. FEATURES Location/Qualifiers source 1..503 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:2260675" /clone_lib="NCI_CGAP_Ov35" /tissue_type="tumor, 5 pooled (see description)" /lab_host="DH10B" /note="Organ: ovary; Vector: pCMV-SPORT6; Site_1: SalI; Site_2: NotI; This library represents the normalized version of NCI_CGAP_Ov23. Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.86 kb. Tumor types include: mixed Mullerian tumor, papillary serous, clear cell, spindle cell. All are primary tumors, metastasis positive. Constructed by Life Technologies." BASE COUNT 94 a 129 c 155 g 125 t ORIGIN 1 tttttttttt tttttttttt tttttttttt tttggcaaat ccccttcttt ccctttaatg 61 aggagggggc tgggggagag gcggaggacc agggaatgca cccatcggag ggttgggttt 121 gggggggggc atctctcaca aaggctgttg gagcccggct cacgtcccac atgtcccttc 181 tcggaccagg gtccagggct gagtcacggg tatcgtcatt ctcaaacctc cactgagccc 241 tgctccctca ggtgttgctg aaggagctgg atgtggtgca acagcttctt ctggtggcca 301 gccagggtga tgcccagggc aggcaggtct tctaggctga gctgacccac atcactgaag 361 gtacagaggc caaacttgga gaagttgtcc tggtagcact ccagtccaat ggctgaaagc 421 caggcctggg gtgagtccag acaaggaaag tccagggcca caggggtcag aagggccctg 481 gaagcccttt ccctgggtcc ccc // LOCUS AI420995 707 bp mRNA linear EST 28-MAR-1999 DEFINITION tf04a06.x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE:2095186 3' similar to TR:O15197 O15197 EPH-FAMILY PROTEIN. ;contains PTR5.t3 MSR1 repetitive element ;, mRNA sequence. ACCESSION AI420995 VERSION AI420995.1 GI:4266926 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 707) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Michael J. Brownstein, M.D., Ph.D., Michael R. Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 970 Std Error: 0.00 Seq primer: -40UP from Gibco High quality sequence stop: 474. FEATURES Location/Qualifiers source 1..707 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:2095186" /clone_lib="NCI_CGAP_Pr28" /sex="male" /dev_stage="adult" /lab_host="DH10B" /note="Organ: prostate; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Plasmid DNA from the normalized library NCI_CGAP_Pr22 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 985608-986759, 1101192-1101959, and 1217928-1220615). Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 140 a 190 c 218 g 155 t 4 others ORIGIN 1 tttgcaaatt cccttctttc cctttaatga ggagggggct ggtggagagg cggaggacca 61 gggaatgcag ccatcggtag ggttgggttt ggtgtggggc atctctcaca gaggctgttg 121 gagcccggct cacgtcccac atgtcccttc tcggaccagt gtccagggct gagtcacggg 181 tatcgtcatt ctcagacctc cactgagccc tgctgcctca ggtgttgctg aaggagctgg 241 atgtggtgca gcagcttctt ctggtggcca gccagggtga tgcccagggc aggcaggtct 301 tctaggctga gctgagccac atcactgaag gtacagaggc caaacttgga gaagttgtcc 361 tggtagcact ccagtccaat ggctgaaagc caggcctggg gtgagtccag acaaggaaag 421 tccagggcca caggggtcag aagggcctgg gaaggccttt cccctgggtc cccgccagcc 481 tgcagggtat ctgggcttgg gatcatcttg tcaaatgcag ccaccagctg gtcaaaatga 541 ggccgccggg cacggtcctt ctgccagtgt ccaacataag tagatgtatc ccagaggaca 601 gcctggaggc gggggcagcc ggaactcctg ctctattgca ttagtacctn ctgctcactc 661 atgtcccagt tnagggcgtc tnncatactc atcactttcc acatgat // LOCUS AI380793 151 bp mRNA linear EST 30-MAR-1999 DEFINITION tg04c03.x1 NCI_CGAP_CLL1 Homo sapiens cDNA clone IMAGE:2107780 3', mRNA sequence. ACCESSION AI380793 VERSION AI380793.1 GI:4190646 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 151) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Ash Alizadeh, John Byrd, M.D., Mike Grever, M.D., Louis M. Staudt, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 227 Std Error: 0.00 Seq primer: -40UP from Gibco. FEATURES Location/Qualifiers source 1..151 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:2107780" /clone_lib="NCI_CGAP_CLL1" /tissue_type="B-cell, chronic lymphotic leukemia" /lab_host="DH10B" /note="Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTGCTTTTTTTTTTTTTTTTTTTTTTTT T 3']; double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo." BASE COUNT 25 a 33 c 52 g 41 t ORIGIN 1 tttttttttg caaattccct tctttccctt taatgaggag ggggctggtg gagaggcgga 61 ggaccaggga atgcagccat cgggagggtt gggtttggtg tggggcatct ctcacagagg 121 ctgttggagc ccggctcacg tcccacatgt c // LOCUS AA815237 340 bp mRNA linear EST 31-DEC-1998 DEFINITION ai64b01.s1 Soares_testis_NHT Homo sapiens cDNA clone 1375561 3' similar to TR:O08644 O08644 MEP. ;, mRNA sequence. ACCESSION AA815237 VERSION AA815237.1 GI:2884833 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 340) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov cDNA Library Preparation: M. Bento Soares, Ph.D., M. Fatima Bonaldo , Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 426 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham. FEATURES Location/Qualifiers source 1..340 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="1375561" /clone_lib="Soares_testis_NHT" /sex="male" /lab_host="DH10B" /note="Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from mRNA obtained from Clontech Laboratories , Inc., and primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization to Cot5, and was constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 60 a 87 c 115 g 78 t ORIGIN 1 tttgcaaatt cccttctttc cctttaatga ggagggggct ggtggagagg cggaggacca 61 gggaatgcag ccatcgggag ggttgggttt ggtgtggggc atctctcaca gaggctgttg 121 gagcccggct cacgtcccac atgtcccttc tcggaccagt gtccagggct gagtcacggg 181 tatcgtcatt ctcagacctc cactgagccc tgctgcctca ggtgttgctg aaggagctgg 241 atgtggtgca gcagcttctt ctggtggcca gccagggtga tgcccagggc aggcaggtct 301 tctaggctga gctgagccac atcactgaag gtacagaggc // LOCUS AI160291 417 bp mRNA linear EST 02-OCT-1998 DEFINITION qb66a12.x1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone IMAGE:1705054 3' similar to TR:O15197 O15197 EPH-FAMILY PROTEIN. ;contains PTR5.t3 MSR1 repetitive element ;, mRNA sequence. ACCESSION AI160291 VERSION AI160291.1 GI:3693671 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 417) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 405. FEATURES Location/Qualifiers source 1..417 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:1705054" /clone_lib="Soares_fetal_heart_NbHH19W" /sex="unknown" /dev_stage="19 weeks" /lab_host="DH10B (ampicillin resistant)" /note="Organ: heart; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by M.Fatima Bonaldo. This library was constructed from the same fetus as the fetal lung library, Soares fetal lung NbHL19W." BASE COUNT 80 a 105 c 139 g 93 t ORIGIN 1 tttttccctt ctttcccttt aatgaggagg gggctggtgg agaggcggag gaccagggaa 61 tgcagccatc gggagggttg ggtttggtgt ggggcatctc tcacagaggc tgttggagcc 121 cggctcacgt cccacatgtc ccttctcgga ccagtgtcca gggctgagtc acgggtatcg 181 tcattctcag acctccactg agccctgctg cctcaggtgt tgctgaagga gctggatgtg 241 gtgcagcagc ttcttctggt ggccagccag ggtgatgccc agggcaggca ggtcttctag 301 gctgagctga gccacatcac tgaaggtaca gaggccaaac ttggagaagt tgtcctggta 361 gcactccagt ccaatggctg aaagccaggc ctggggtgag tccagacaag gaaagtc // LOCUS AI141423 399 bp mRNA linear EST 05-OCT-1998 DEFINITION qa67a04.x1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone IMAGE:1691790 3' similar to TR:O15197 O15197 EPH-FAMILY PROTEIN. ;contains PTR5.t3 MSR1 repetitive element ;, mRNA sequence. ACCESSION AI141423 VERSION AI141423.1 GI:3648880 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 399) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 1381 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 398. FEATURES Location/Qualifiers source 1..399 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:1691790" /clone_lib="Soares_fetal_heart_NbHH19W" /sex="unknown" /dev_stage="19 weeks" /lab_host="DH10B (ampicillin resistant)" /note="Organ: heart; Vector: pT7T3D (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was size selected, ligated to Eco RI adapters (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of a modified pT7T3 vector (Pharmacia). Library went through one round of normalization to a Cot = 5. Library constructed by M.Fatima Bonaldo. This library was constructed from the same fetus as the fetal lung library, Soares fetal lung NbHL19W." BASE COUNT 72 a 101 c 129 g 97 t ORIGIN 1 tttttttttt ttcccttctt tccctttaat gaggaggggg ctggtggaga ggcggaggac 61 cagggaatgc agccatcggg agggttgggt ttggtgtggg gcatctctca cagaggctgt 121 tggagcccgg ctcacgtccc acatgtccct tctcggacca gtgtccaggg ctgagtcacg 181 ggtatcgtca ttctcagacc tccactgagc cctgctgcct caggtgttgc tgaaggagct 241 ggatgtggtg cagcagcttc ttctggtggc cagccagggt gatgcccagg gcaggcaggt 301 cttctaggct gagctgagcc acatcactga aggtacagag gccaaacttg gagaagttgt 361 cctggtagca ctccagtcca atggctgaaa gccaggcct // LOCUS AA913460 447 bp mRNA linear EST 10-JUN-1998 DEFINITION ol30g02.s1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE:1525010 3' similar to TR:O15197 O15197 EPH-FAMILY PROTEIN. ;, mRNA sequence. ACCESSION AA913460 VERSION AA913460.1 GI:3052852 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 447) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Insert Length: 544 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 414. FEATURES Location/Qualifiers source 1..447 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:1525010" /clone_lib="Soares_NFL_T_GBC_S1" /lab_host="DH10B" /note="Organ: pooled; Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; Equal amounts of plasmid DNA from three normalized libraries (fetal lung NbHL19W, testis NHT, and B-cell NCI_CGAP_GCB1) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 297480-302087, 682632-687239, 726408-728711, and 729096-731399. Subtraction by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 89 a 109 c 152 g 96 t 1 others ORIGIN 1 ttttgcaaat tcccttcttt ccctttaatg aggagggggc tggtggagag gcggaggacc 61 agggaatgca gccatcggga gggttgggtt tggtgtgggg catctctcac agaggctgtt 121 ggagcccggc tcacgtccca catgtccctt ctcggaccag tgtccagggc tgagtcacgg 181 gtatcgtcat tctcagacct ccactgagcc ctgctgcctc aggtgttgct gaaggagctg 241 gatgtggtgc agcagcttct tctggtggcc agccagggtg atgcccaggg caggcaggtc 301 ttctaggctg agctgagcca catcactgaa ggtacagagg ccaaacttgg agaagttgtc 361 ctggtagcac tccagtccaa tggctgaaac aggncctggg gtgagtcaga caaggaaagt 421 caggggcaca ggggtcagaa gggcctg // LOCUS AA903405 72 bp mRNA linear EST 09-JUN-1998 DEFINITION ok62c10.s1 NCI_CGAP_GC4 Homo sapiens cDNA clone IMAGE:1518546 3' similar to TR:O15197 O15197 EPH-FAMILY PROTEIN. ;, mRNA sequence. ACCESSION AA903405 VERSION AA903405.1 GI:3038528 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 72) AUTHORS NCI-CGAP http://www.ncbi.nlm.nih.gov/ncicgap. TITLE National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index JOURNAL Unpublished (1997) COMMENT Contact: Robert Strausberg, Ph.D. Email: cgapbs-r@mail.nih.gov Tissue Procurement: Christopher A. Moskaluk, M.D., Ph.D., Michael Emmert-Buck, M.D., Ph.D. cDNA Library Preparation: M. Bento Soares, Ph.D. cDNA Library Arrayed by: Greg Lennon, Ph.D. DNA Sequencing by: Washington University Genome Sequencing Center Clone distribution: NCI-CGAP clone distribution information can be found through the I.M.A.G.E. Consortium/LLNL at: www-bio.llnl.gov/bbrp/image/image.html Insert Length: 346 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 1. FEATURES Location/Qualifiers source 1..72 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:1518546" /clone_lib="NCI_CGAP_GC4" /tissue_type="pooled germ cell tumors" /lab_host="DH10B" /note="Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; 1st strand cDNA was prepared from 3 pooled germ cell tumors, and was then primed with a Not I - oligo(dT) primer. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library is normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 9 a 20 c 24 g 19 t ORIGIN 1 cctccactga gccctgctgc ctcaggtgtt gctgaaggag ctggatgtgg tgcagcagct 61 tcttctggtg gc // LOCUS AA984196 440 bp mRNA linear EST 27-MAY-1998 DEFINITION am82c03.s1 Stratagene schizo brain S11 Homo sapiens cDNA clone IMAGE:1629604 3' similar to gb:M59371 TYROSINE-PROTEIN KINASE RECEPTOR ECK PRECURSOR (HUMAN);, mRNA sequence. ACCESSION AA984196 VERSION AA984196.1 GI:3162721 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 440) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin ,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-NCI human EST Project JOURNAL Unpublished (1997) COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Possible reversed clone: polyT not found Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 419. FEATURES Location/Qualifiers source 1..440 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:1629604" /clone_lib="Stratagene schizo brain S11" /sex="male" /tissue_type="schizophrenic brain S-11 frontal lobe" /dev_stage="34 years old" /lab_host="SOLR (kanamycin resistant)" /note="Vector: Bluescript SK-; Site_1: EcoRI; Library constructed from S-11 frontal lobe, male, 34 years old, 50% caucasian, 50% Aleutian. Schizophrenic suicide. Random primed into EcoRI site of ZAP II Vector. Mass excised. Custom library. Avg insert length 1.4kb. Material obtained by Johnston N., Torrey, E.F., Yolken R., and the Stanley Neuropathology Consortium - Analysis of RNAs from the Brains of Individuals with Psychiatric Diseases (Unpublished) Stanley Neurovirology Laboratory, Johns Hopkins School of Medicine, Baltimore MD." BASE COUNT 76 a 130 c 149 g 82 t 3 others ORIGIN 1 gtgtcaggcc atccgagaac ttgcccggga agtcgatcct gcttatatca agattgagga 61 ggtcattggg acaggctctt ttggagaagt gcgccagggc cgcctgcagc cacggggacg 121 gagggagcag actgtggcca tccaggcact gtggagngnn gcgccgaaag cctgcagatg 181 accttcctgg gccgggcgca gtgctgggtc agttccagca ccccaacatc ctgcggctgg 241 agggcgtggt caccaagagc cgacccctca tggtgctgac ggagttcatg gagcttggcc 301 ccctggacag cttcctcagg cagcgggagg gccagttcag cagcctgcag ctggtggcca 361 tgcagcgggg agtggctgct gcatgcagta cctgtccagc tttgccttcg tccatcgctc 421 gctgtctgcc cacagcgtgc // LOCUS AA609284 394 bp mRNA linear EST 02-MAR-1998 DEFINITION af13e05.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1031552 3' similar to TR:G758789 G758789 TYROSINE KINASE CEK9 RECEPTOR ;, mRNA sequence. ACCESSION AA609284 VERSION AA609284.1 GI:2457712 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 394) AUTHORS Hillier,L., Allen,M., Bowles,L., Dubuque,T., Geisel,G., Jost,S., Krizman,D., Kucaba,T., Lacy,M., Le,N., Lennon,G., Marra,M., Martin ,J., Moore,B., Schellenberg,K., Steptoe,M., Tan,F., Theising,B., White,Y., Wylie,T., Waterston,R. and Wilson,R. TITLE WashU-NCI human EST Project JOURNAL Unpublished (1997) COMMENT Contact: Wilson RK Washington University School of Medicine 4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108 Tel: 314 286 1800 Fax: 314 286 1810 Email: est@watson.wustl.edu This clone is available royalty-free through LLNL ; contact the IMAGE Consortium (info@image.llnl.gov) for further information. Possible reversed clone: similarity on wrong strand Insert Length: 620 Std Error: 0.00 Seq primer: -40m13 fwd. ET from Amersham High quality sequence stop: 380. FEATURES Location/Qualifiers source 1..394 /organism="Homo sapiens" /db_xref="taxon:9606" /clone="IMAGE:1031552" /clone_lib="Soares_testis_NHT" /sex="male" /lab_host="DH10B" /note="Vector: pT7T3D-Pac (Pharmacia) with a modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st strand cDNA was prepared from mRNA obtained from Clontech Laboratories , Inc., and primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCAATTTTTTTTTTTTTTTTT 3']. Double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. Library went through one round of normalization to Cot5, and was constructed by Bento Soares and M. Fatima Bonaldo. " BASE COUNT 74 a 98 c 128 g 94 t ORIGIN 1 ttttttttgc aaattccctt ctttcccttt aatgaggagg gggctggtgg agaggcggag 61 gaccagggaa tgcagccatc gggagggttg ggtttggtgt ggggcatctc tcacagaggc 121 tgttggagcc cggctcacgt cccacatgtc ccttctcgga ccagtgtcca gggctgagtc 181 acgggtatcg tcattctcag acctccactg agccctgctg cctcaggtgt tgctgaagga 241 gctggatgtg gtgcagcagc ttcttctggt ggccagccag ggtgatgccc agggcaggca 301 ggtcttctag gctgagctga gccacatcac tgaaggtaca gaggccaaac ttggagaagt 361 tgtcctggta gcactccagt ccaatggctg aaag // LOCUS T09277 398 bp mRNA linear EST 03-AUG-1993 DEFINITION EST07170 Infant Brain, Bento Soares Homo sapiens cDNA clone HIBBR66 5' end similar to Tyrosine kinase, mRNA sequence. ACCESSION T09277 VERSION T09277.1 GI:390305 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 398) AUTHORS Adams,M.D., Soares,M.B., Kerlavage,A.R., Fields,C. and Venter,J.C. TITLE Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library JOURNAL Nat. Genet. 4, 373-380 (1993) MEDLINE 94004965 COMMENT Other_ESTs: EST07169 Contact: Adams, MD The Institute for Genomic Research 932 Clopper Road, Gaithersburg, MD 20878 Tel: 3018699056 Fax: 3018699423 Email: mdadams@tigr.org Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..398 /organism="Homo sapiens" /db_xref="ATCC (inhost):85735" /db_xref="taxon:9606" /clone="HIBBR66" /clone_lib="Infant Brain, Bento Soares" BASE COUNT 71 a 115 c 140 g 68 t 4 others ORIGIN 1 aggccatccg agaacttgcc cgggaagtcg atcctgctta tatcaagatt gaggaggtca 61 ttgggacagg ctcttttgga gaagtgcgcc agggccgcct gcagccacgg ggacggaggg 121 agcagactgt ggccatccag gccctgtggg cggngnggcg ccgaaagcct gcagatgacc 181 ttcctgggcc gggccgcagt gctgggtcag ttccagcacc ccaacatcct gcggctggag 241 ggcgtggtca ccaagagccg acccctcatg gtgctgacgg agttcatgga gcttngcccc 301 ctggacagct tcctcaggca gcgggagggc cagttcagca gcctgnagct ggtggccatg 361 cagcggggag tggctgctgc catgcagtac ctgtccag // LOCUS T09276 241 bp mRNA linear EST 03-AUG-1993 DEFINITION EST07169 Infant Brain, Bento Soares Homo sapiens cDNA clone HIBBR66 3' end, mRNA sequence. ACCESSION T09276 VERSION T09276.1 GI:390304 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 241) AUTHORS Adams,M.D., Soares,M.B., Kerlavage,A.R., Fields,C. and Venter,J.C. TITLE Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library JOURNAL Nat. Genet. 4, 373-380 (1993) MEDLINE 94004965 COMMENT Other_ESTs: EST07170 Contact: Adams, MD The Institute for Genomic Research 932 Clopper Road, Gaithersburg, MD 20878 Tel: 3018699056 Fax: 3018699423 Email: mdadams@tigr.org Seq primer: M13-21. FEATURES Location/Qualifiers source 1..241 /organism="Homo sapiens" /db_xref="ATCC (inhost):85735" /db_xref="taxon:9606" /clone="HIBBR66" /clone_lib="Infant Brain, Bento Soares" BASE COUNT 40 a 57 c 81 g 57 t 6 others ORIGIN 1 gcaaattccc ttctttccct ttaatgagga gggggctggt ggagaggcgg aggaccaggg 61 antgcagcca tcgggagggt tgggtttggt gtggggcatc tctcacagag gctgttggag 121 cccggntcac gtcccacatg tcccttntcg gaccagtgtc cagggntgag tcacgggtat 181 cgtcattntc agacctccac tgagccctgn tgcctcaggt gttgctgaag gagctggatg 241 t // LOCUS T08512 387 bp mRNA linear EST 03-AUG-1993 DEFINITION EST06404 Infant Brain, Bento Soares Homo sapiens cDNA clone HIBBF52 5' end similar to Tyrosine kinase, mRNA sequence. ACCESSION T08512 VERSION T08512.1 GI:389540 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 387) AUTHORS Adams,M.D., Soares,M.B., Kerlavage,A.R., Fields,C. and Venter,J.C. TITLE Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library JOURNAL Nat. Genet. 4, 373-380 (1993) MEDLINE 94004965 COMMENT Other_ESTs: EST06403 Contact: Adams, MD The Institute for Genomic Research 932 Clopper Road, Gaithersburg, MD 20878 Tel: 3018699056 Fax: 3018699423 Email: mdadams@tigr.org Seq primer: M13 Reverse. FEATURES Location/Qualifiers source 1..387 /organism="Homo sapiens" /db_xref="ATCC (inhost):85034" /db_xref="taxon:9606" /clone="HIBBF52" /clone_lib="Infant Brain, Bento Soares" BASE COUNT 71 a 110 c 137 g 65 t 4 others ORIGIN 1 acgaggaccc ctgtcaggcc atccgagaac ttgcccggga agtcgatcct gcttatatca 61 agattgagga ggtcattggg acaggctctt ttggagaagt gcgccagggc cgcctgcagc 121 cacggggacg gagggagcag actgtggcca tccaggccct gtgggcggng aggcgccgaa 181 agcctgcaga tgaccttcct gggccgggcc gcagtgctgg gtcagttcca gcaccccaac 241 atcctgcggc tggagggcgt ggtcaccaag agccgacccc tcatggtgct gacggagttc 301 atggagcttg gccccctgga cagattnctc aggcgggagg gccagttcag cagcctgcag 361 ctngtngcca tgcagcgggg gagttgg // LOCUS T08511 345 bp mRNA linear EST 03-AUG-1993 DEFINITION EST06403 Infant Brain, Bento Soares Homo sapiens cDNA clone HIBBF52 3' end similar to Tyrosine kinase, mRNA sequence. ACCESSION T08511 VERSION T08511.1 GI:389539 KEYWORDS EST. SOURCE human. ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 345) AUTHORS Adams,M.D., Soares,M.B., Kerlavage,A.R., Fields,C. and Venter,J.C. TITLE Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library JOURNAL Nat. Genet. 4, 373-380 (1993) MEDLINE 94004965 COMMENT Other_ESTs: EST06404 Contact: Adams, MD The Institute for Genomic Research 932 Clopper Road, Gaithersburg, MD 20878 Tel: 3018699056 Fax: 3018699423 Email: mdadams@tigr.org Seq primer: M13-21. FEATURES Location/Qualifiers source 1..345 /organism="Homo sapiens" /db_xref="ATCC (inhost):85034" /db_xref="taxon:9606" /clone="HIBBF52" /clone_lib="Infant Brain, Bento Soares" BASE COUNT 64 a 80 c 112 g 78 t 11 others ORIGIN 1 caaattccct tctttccctt taatgaggag ggggctggtg gagaggcgga ggaccaggga 61 atgcagccat cgggagggtt gggtttggtg tggggcatct ntcacagagg ctnttggagc 121 ccggntcacg tcccacatgt cccttntcgg accagtntcc agggntgagt cacgggtatc 181 gtcattntca gacctccact gagccctgct gcctcaggtg ttgctgaagg agctggatgt 241 ggtgcagnan ntttttctgg tggccagcca gggtgatgcc cagggcaggc aggtcttnta 301 ggctgagctg agccacatca ctgaaggtac aagagggcca aactt //